The impact of fluid-dynamic stress in stirred tank bioreactors on the synthesis of cellulases by Trichoderma reesei at the intracellular and extracellular levels

Cellulases for bioethanol production are mainly made by fed-batch fermentation using a filamentous fungus, Trichoderma reesei. Agitation at different scales impacts on morphology, rheology and growth rate can be correlated EDCF?max. Typically, EDCF?max is much smaller commercial scale fungal size, viscosity greater. Here, to increase understanding, continuous culture in 3 L bioreactors two values were undertaken. The higher decreased the cellulase (concentration, 21%; specific rate, 24%; protein yield, 20%) whilst proteomic analysis showed, an intracellular level, decrease of hemicellulase synthesis. An stress proteins also occurred, which may help cells limit impact fluid dynamic stress. Also, during bench varied with similarly that between culture.